Before medication, the patient was pathologically diagnosed with esophageal carcinoma (squamous cell carcinoma). Due to the lack of surgical indications, surgery was not feasible. Subsequently, CHA for injection was used in combination with radiotherapy and chemotherapy. After completion nine courses of chemotherapy, CHA for injection alone was used until now. After 20 days of mediation, the imaging examination showed a reduction in the lesion size. A follow-up examination after 2 years and 7 months of mediation indicated little change in the esophageal lumen. During the period of combined medication, the radiotherapy and chemotherapy basically had no side effects. Since the mediation, the patient has been able to travel away. Up to now, the patient’s condition is stable and improving, with no signs of recurrence or metastasis, normal blood biochemical indicators, and good state of life.
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Chlorogenic acid inhibits esophageal squamous cell carcinoma growth in vitro and in vivo by downregulating the expression of BMI1 and SOX2
Chlorogenic acid inhibits esophageal squamous cell carcinoma growth in vitro and in vivo by downregulating the expression of BMI1 and SOX22019.10.26In this study, we confirmed the inhibition of proliferation by CHA in ESCC cells, as well as the reduction of ESCC xenograft volume by CHA in vivo. In addition, CHA also suppressed both the migration and invasion of ESCC cells in vitro. In a carcinogen-induced murine model of ESCC, hyperplasia of the esophagus was slowed by CHA, while mice suffering from ESCC that were treated with CHA had longer survival times than mice in the control group. The measurement of pluripotency factors (BMI1, SOX2, OCT4 and Nanog) that are related to poor prognosis revealed reduced expression of both BMI1 and SOX2, but not of OCT4 or Nanog, in ESCC cells, in both a dose- and time-dependent manner. -
Chlorogenic acid down-regulates the expression of PD-L1 in esophageal squamous cell carcinoma via IFN-γ signaling pathway
Chlorogenic acid down-regulates the expression of PD-L1 in esophageal squamous cell carcinoma via IFN-γ signaling pathway2021.02.09In this study, the regulatory effects of CHA on the expression of programmed cell death ligand 1 (PD-L1) in esophageal squamous cell carcinoma (ESCC), as well as the role of interferon γ (IFN-γ), has been discussed using both in vitro and in vivo animal models. ESCC murine model was established according to the standard operating procedures (SOP) of Animal Experiment Center of Institute of Materia Medica, Chinese Academy of Medical Sciences. The expression of PD-L1 in esophageal tissues of murine models was analyzed using the microarray assay. Then, the results were verified by qRT-PCR, Western blot and immunohistochemistry (IHC) staining, the molecular mechanism was explored in KYSE180 and KYSE510 ESCC cells in vitro. The results showed that CHA could suppress the expression of PD-L1 in tumor tissues in murine models significantly, rather than the expression in KYSE180 and KYSE510 ESCC cells in vitro. However, after the pretreatment of IFN-γ, the expression of PD-L1 was significantly increased, then it was down-regulated by CHA in both doseand time-dependent manner. Meanwhile, the expression of interferon regulatory factor 1 (IRF1), an upstream regulatory factor of PD-L1, was suppressed by CHA in both KYSE180 and KYSE510 pretreated with IFN-γ, which was consistent with the expression of PD-L1. These results indicate that CHA down-regulates the expression of PD-L1 in ESCC via IFN-γ-IRF1 signaling pathway, providing the molecular theoretical basis for exploration of new treatment of ESCC.
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Chlorogenic acid suppresses colony formation and invasion of esophageal squamous cell carcinoma via down-regulating the expression of Notch1
Chlorogenic acid suppresses colony formation and invasion of esophageal squamous cell carcinoma via down-regulating the expression of Notch12021.06.16This study aims to investigate the effect and mechanism of Notch1 in the process of CHA suppressing esophageal squamous cell carcinoma (ESCC).