Our company assembled its technical backbones, gave full play to its scientific research advantages, and after long-term research, with the strenuous efforts of the scientific research team led by Wang Li, Yang Huarong, and Wang Zhu, a scientific and detailed experiment on the sensitization of CHA was carried out. And a paper titled "Comprehensive Evaluation of the Non-sensitizing Property of CHA" was published in the authoritative journal "Chinese Journal of Medicinal Guide", which aroused a strong response from relevant people. It opened up new ideas and windows for the treatment and control of cancer and brought good news to cancer patients.

The following is an excerpt from the paper:

Objective: Through the sensitization tests of CHA with different purities, the sensitization tests of CHA with different purities after removing macromolecules by equilibrium dialysis, the sensitization test of the macromolecular substances obtained by dialysis, the separation and identification of the sensitizing dialysate by LC-MS, and the comparative analysis of the domestic and foreign literatures on the sensitization research of CHA, this paper comprehensively studies and demonstrates whether CHA has allergenicity.
Results: No allergic reactions occurred in the CHA extracts with a purity higher than 92%, the injections, the expired injections, the degraded injections, the external dialysate, and the internal dialysate; Macromolecular compounds were identified by LC-MS in the CHA extracts with a purity lower than 40% and their internal dialysates, and obvious allergic reactions occurred.
Conclusion: The test results prove that CHA is not an allergen, and this conclusion is consistent with the conclusions drawn from clinical studies abroad in the 1960s and at the end of the 1970s.
Fang-hua Huang and others further studied the anaphylactoid reaction of CHA and its mechanism from the perspective of anaphylactoid reactions. The author conducted the test of the degranulation (DI%) of peritoneal mast cells in guinea pigs induced by a high dose of CHA and analyzed the degranulation of RBL-2H3 cells induced by CHA. It shows that CHA can cause the degranulation of mast cells, but the degranulation index (DI%) did not increase significantly compared with the control group. The author suggests that the reaction caused by CHA under these experimental conditions should be an anaphylactoid reaction, and an anaphylactoid reaction specifically refers to the toxic symptoms similar to anaphylaxis caused by the stimulation of the body by external substances (drugs, X-rays, etc.). The administration of a high dose of CHA used in the literature can be regarded as a stress response of the peritoneal mast cells of guinea pigs under the action of a high dose of CHA, rather than an allergic reaction. The conclusion of this literature is that the degranulation effect of CHA on guinea pig mast cells and RBL-2H3 cells is one of the mechanisms of the anaphylactoid reaction of a high dose of CHA. An anaphylactoid reaction is a controllable adverse reaction (reducing the dosage or slowing down the administration speed).

[Keywords] CHA; Sensitization; Equilibrium dialysis method; LC-MS; Macromolecules
[Chinese Library Classification Number] R285.5 [Document Code] A [Article Number] 1009-0959(2010)12-2xxx-0x
For the detailed paper, please refer to "Chinese Journal of Medicinal Guide", Volume 12, Issue 12, 2010, page 2115, "Comprehensive Evaluation of the Non-sensitizing Property of CHA".